List of Buffer Recipes
50mM Sodium Acetate pH5
0.1M Borate Buffer pH9
1M ethanolamine pH9
1M Tris-HCl pH 7-9
50mM Sodium Acetate pH5
7.4 ml of 0.2M acetic acid (60.05 g/mol) + 17.6 ml of 0.2M sodium acetate (82.03 g/mol) made up with water to a total volume of 100 ml will have a pH of 5.
0.1M Sodium bicarbonate buffer pH10.2
8.4g Sodium bicarbonate (84.01 g/mol) + 10.6g Sodium carbonate (105.99 g/mol) in 1L pH10.2
0.1M Borate Buffer pH9
6.18g Boric acid (61.83 g/mol) + 1.3g NaOH (40.00g/mol) in 1L pH9.1
Boric Acid Mr = 61.83 0.1M = 6.18mg/mL (3.09g in 500mL)
Adjust pH with 4mL 4M NaOH (160mg/mL) (640mg)
1M ethanolamine pH9
6mL ethanolamine per 100mL PBS
Adjust pH with conc. HCL (pH 8 - 11)
Example for 20mL: (1.2mL ethanolamine in 17.5mL PBS + 1.3mL 30% HCL) pH9
1M Tris-HCl pH 7-9
tris(hydroxymethyl)aminomethane (Tris) has a pKa of 8.06 so Tris buffer can made at pH 7-9.
121.1 g Tris base
800 ml Milli-Q H2O
Dissolve Tris in H2O and adjust pH with conc. HCL (pH 7.4 ~70mL, 8.0 ~42mL, 8.5 ~20mL, 9.0 ~8mL)
Make up solution to 1L with H2O
Sterile filter if required.